YAYINLAR
Association of Polymorphisms in TCF7L2 Gene with Gastric Cancer Risk: A Preliminary Study in Turkish
Dudu Erkoç Kaya , Hilal Arıkoğlu , İncilay Celik Sümen , Ebru Avcı , Özlem Ata , Emine Arslan
Abstract Aim: The Transcription factor 7-like 2 (TCF7L2) gene, located on chromosome 10q25.3, encodes a transcription factor, which contains a high mobility group box, demonstrated in association with many cancer types. This factor is a critical part of Wnt/β-catenin signaling pathway that plays key roles in regulation of embryonic development and homeostasis in mature tissues. It is known that the constant activation of Wnt/β-catenin signaling pathway can cause cancer development. In this study, it is aimed to reveal the association between rs7903146C>T and rs12255372G>T single nucleotide polymorphisms in TCF7L2 gene, the key effector of Wnt/β-catenin signaling pathway, and gastric cancer and to determine associated risk alleles. Material and Method: In our study, polymorphisms in TCF7L2 gene were genotyped using PCR-PFLP technique in 38 patients with gastric cancer and 48 healthy individuals. The obtained data were statistically analyzed and pT and disease (p> 0.05) while 12255372G>T polymorphism was associated with the disease under additive model (OR: 0.366 [95% CI: 0.135-0.989] p=0.047). Discussion: This is the first study to examine the association between TCF7L2 gene and gastric cancer risk in Turkish population and suggests that rs12255372G>T could be a potential indicator for gastric cancer. On the other hand, further studies are required which will be carry out in more increased number of samples in a wider population.
Keywords TCF7L2 Gene; SNP; Gastric Cancer; Turkish
THE ASSOCİATİON OF METHYLENETETRAHYDROFOLATE REDUCTASE (MTHFR) AND THYMİDYLATE SYNTHASE (TS) GENE POLYMORPHİSMS WİTH GASTRİC CANCER
INCİLAY CELİK SUMEN , HİLAL ARİKOGLU , EMİNE ARSLAN, OZLEM ATA, AYSEL KIYICI, SEYİT ALİ KAYİS
Abstract: Objective: In our study, the relationship between C677T and tandem repeat sequence polymorphisms in the genes coding MTHFR and TS enzymes respectively, which play a key role in folate metabolism and the folate level in serum as a phenotypic indicator of these changes and stomach cancer was investigated.
Method: 38 patients who were diagnosed with stomach cancer in the Oncology Department at Selcuk University Faculty of Medicine and 48 healthy individuals were included in the study. MTHFR and TS gene polymorphisms were analyzed using the PCR-RFLP and PCR-agarose gel electrophoresis methods respectively.
Result: No statistically significant relationship was found between the C/C, C/T and T/T genotypes in the position 677 of MTHFR gene and stomach cancer (p˃0.05). It was found that there was a relationship between 2R/3R and 3R/3R genotypes which occur as a result of tandem repeat polymorphisms (R: 28 base pair) in the 5ˈUTR enhancer region of the TS gene and stomach cancer and that the 3R allele was a risk allele (p˂0.05). On the other hand, the amount of folate in serum was found to be higher in patients in comparison to the control group. Moreover, it was detected that stomach cancer was not correlated with age and gender.
Discussion: In conclusion, it is thought that TS polymorphism may play an important part in the development of stomach cancer by affecting folate metabolism.
Keywords: Gastric cancer, Polymorphism, Methylenetetrahydrofolate Reductase (MTHFR), Thymidylate Synthase (TS)